Analysis of simple sugars and sorbitol in fruit by high-performance liquid chromatography
Michael L. Richmond; Sebastiao C. C. Brandao; J. Ian Gray; Pericles Markakis; Charles M. Stine
Journal of Agricultural and Food Chemistry. 1981;29(1):4-7.
Abstract
The application of a high-performance liquid chromatographic (LC) procedure for the determination of sugars and sorbitol in fresh fruits is described. This system combines the use of two bonded phase carbohydrate columns, joined in tandem; a ternary mobile phase (acetonitrile-water-ethanol) and a differential refractometer to accurately and precisely separate fructose, glucose, sorbitol, sucrose, and maltose. Total analysis time was 20 min for the five-sugar mixture. Twenty-four fruits were analyzed including eleven from the family Rosaceae, which often contain sorbitol. Sample recoveries ranged from 98% for fructose to 102% for maltose. © 1981 American Chemical Society.
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